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KMID : 1005220170320030147
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Journal of Embryo Transfer
2017 Volume.32 No. 3 p.147 ~ p.151
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Study on Ovum-pick up for Improvement of Embryo Transfer Efficiency in Hanwoo Cows
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Cho Sang-Rae
Kang Sung-Sik
Kim Ui-Hyung
Lee Suk-Dong
Lee Myoung-Sook
Yang Byoung-Chul
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Abstract
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Commercial applications of OPU/IVP were to produce embryos and calves from high genetic cows. The aim of this present study was to compare the number of recovered oocytes and cultured In vitro produced embryos from Ovum Pick-up (OPU). OPU derived embryo production was carried out of oocytes by ultrasonographic guided follicular aspiration and then produced in vitro produced blastocysts by IVP culture system. In result, the rate of recovered oocytes was obtained 612 (57.2%) and 451(73.7) G1+G2 grade oocytes. No difference of recovered rate (51.1~62.1%) was seen in six donor. The rate of cleavage and blastocyst development were obtained 320 (70.9%) and 78 (24.4%) that was 3.3±0.4 cleaved embryo and 0.9±0.2 blastocysts per session. Cleavage rate of OPU oocytes in No. 6 donor was 90.6%, significantly (P<0.05) higher than that in the other donors, However, blastocysts was similar (25.8~30.0%). In conclusion, limited numbers of OPU oocytes had competent development when cultured in SOF culture medium.
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KEYWORD
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In vivo oocytes , OPU , Embryo
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